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genotypingSelf-Collected, Ambient Temperature–Preserved Nasal Swabs Compared to Samples Collected by Trained Staff for Genotyping
JMIR Form Res 2023;7:e32848
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Samples will be tested with the Anyplex TM II HP28 Detection kit (Seegene), a semi-qualitative test for the detection of DNA and genotyping of HPV types. DNA extraction will be performed using the STARMag Universal extraction kit (Eurobio Scientific) on the STARlet (Seegene) automated system. After extraction, genotyping will be done by amplification on a CFX96 TM thermocycler (Bio Rad).
JMIR Res Protoc 2023;12:e47379
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SNP genotyping was performed using the commercial Flex Six Genotyping integrated fluidic circuit kit (Fluidigm). This method is based on allele-specific polymerase chain reaction detection using an integrated fluidic circuit.
JMIR Dermatol 2023;6:e39567
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