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The biochemically verified smoking status of the study population (ie, serum cotinine) will be correlated with serological data and COVID-19 outcomes (ie, clinical symptoms and hospitalization).
Epidemiological exposure data and venous blood (for measurements of anti–SARS-Co V-2–specific Ig G and serum cotinine levels) will be systematically collected.
JMIR Res Protoc 2021;10(11):e32285
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Baseline and follow-up urine samples were analyzed for cotinine by liquid chromatography–tandem mass spectrometry [20]. Cotinine, the main proximate metabolite of nicotine, has a half-life of about 16 hours [19] and is a biomarker of ongoing or recent exposure (past 5-6 days). The limit of quantitation (LOQ) for cotinine was 0.05 ng/m L. Cotinine cutpoints were 0.05-30 ng/m L for light or heavy SHS and >30 ng/m L for active smoking or vaping [5].
JMIR Form Res 2020;4(8):e18583
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