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The sample size was determined based on a small effect size of f=0.1 for the mixed ANOVA model with repeated measures (4 time points) and interactions with the Age Group factor (3 categories), given an error probability of α=.05 and a power of 0.95, resulting in a sample size of 264 individuals (determined with G*Power, a free-source statistical software package) [44]. However, having a larger number of subjects is desirable owing to expected dropouts over the long duration of the study.
JMIR Res Protoc 2022;11(3):e32352
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