<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD Journal Publishing DTD v2.0 20040830//EN" "journalpublishing.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="2.0" xml:lang="en" article-type="research-article"><front><journal-meta><journal-id journal-id-type="nlm-ta">JMIR Form Res</journal-id><journal-id journal-id-type="publisher-id">formative</journal-id><journal-id journal-id-type="index">27</journal-id><journal-title>JMIR Formative Research</journal-title><abbrev-journal-title>JMIR Form Res</abbrev-journal-title><issn pub-type="epub">2561-326X</issn><publisher><publisher-name>JMIR Publications</publisher-name><publisher-loc>Toronto, Canada</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">v10i1e83556</article-id><article-id pub-id-type="doi">10.2196/83556</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Paper</subject></subj-group></article-categories><title-group><article-title>Increasing Physical Activity in Educational Settings Using Mixed Reality Technology: Iterative Formative Study</article-title></title-group><contrib-group><contrib contrib-type="author"><name name-style="western"><surname>Spring</surname><given-names>Katherine E</given-names></name><degrees>PhD</degrees><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Feldt</surname><given-names>Calvin W</given-names></name><degrees>BS</degrees><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Posey</surname><given-names>Matthew Parker</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Nauta</surname><given-names>Phillip</given-names></name><degrees>MS</degrees><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Chen</surname><given-names>Senlin</given-names></name><degrees>PhD</degrees><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Shepherd</surname><given-names>David C</given-names></name><degrees>PhD</degrees><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><name name-style="western"><surname>Staiano</surname><given-names>Amanda E</given-names></name><degrees>PhD</degrees><xref ref-type="aff" rid="aff1">1</xref></contrib></contrib-group><aff id="aff1"><institution>Pennington Biomedical Research Center</institution><addr-line>6400 Perkins Rd.</addr-line><addr-line>Baton Rouge</addr-line><addr-line>LA</addr-line><country>United States</country></aff><aff id="aff2"><institution>Division of Computer Science and Engineering, Louisiana State University</institution><addr-line>Baton Rouge</addr-line><addr-line>LA</addr-line><country>United States</country></aff><aff id="aff3"><institution>School of Kinesiology, Louisiana State University</institution><addr-line>Baton Rouge</addr-line><addr-line>LA</addr-line><country>United States</country></aff><contrib-group><contrib contrib-type="editor"><name name-style="western"><surname>Mavragani</surname><given-names>Amaryllis</given-names></name></contrib></contrib-group><contrib-group><contrib contrib-type="reviewer"><name name-style="western"><surname>Andriyani</surname><given-names>Fitria Dwi</given-names></name></contrib></contrib-group><author-notes><corresp>Correspondence to Amanda E Staiano, PhD, Pennington Biomedical Research Center, 6400 Perkins Rd., Baton Rouge, LA, 70808, United States, 1 2257632729; <email>Amanda.Staiano@pbrc.edu</email></corresp></author-notes><pub-date pub-type="collection"><year>2026</year></pub-date><pub-date pub-type="epub"><day>25</day><month>3</month><year>2026</year></pub-date><volume>10</volume><elocation-id>e83556</elocation-id><history><date date-type="received"><day>04</day><month>09</month><year>2025</year></date><date date-type="accepted"><day>23</day><month>02</month><year>2026</year></date></history><copyright-statement>&#x00A9; Katherine E Spring, Calvin W Feldt, Matthew Parker Posey, Phillip Nauta, Senlin Chen, David C Shepherd, Amanda E Staiano. Originally published in JMIR Formative Research (<ext-link ext-link-type="uri" xlink:href="https://formative.jmir.org">https://formative.jmir.org</ext-link>), 25.3.2026. </copyright-statement><copyright-year>2026</copyright-year><license license-type="open-access" xlink:href="https://creativecommons.org/licenses/by/4.0/"><p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">https://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in JMIR Formative Research, is properly cited. The complete bibliographic information, a link to the original publication on <ext-link ext-link-type="uri" xlink:href="https://formative.jmir.org">https://formative.jmir.org</ext-link>, as well as this copyright and license information must be included.</p></license><self-uri xlink:type="simple" xlink:href="https://formative.jmir.org/2026/1/e83556"/><abstract><sec><title>Background</title><p>As physical education classes are lost to budget cuts and recess is canceled to meet standardized testing goals, the modern school day has become dominated by sedentary digital activities. To reverse this trend, current interventions have focused on reducing screen time. However, instead of fighting this digital invasion, this study examined the use of technology, specifically mixed reality, to turn screen time from sedentary into active time, promoting physical activity in a classroom setting.</p></sec><sec><title>Objective</title><p>The primary aim of this study was to iteratively develop and test a mixed reality prototype that promotes physical activity (eg, jumping, squatting, and punching) during a digital classroom activity. The primary outcomes were the percentage of active time during the activity, a breakdown of the intensity of that active time, and an evaluation of the prototype&#x2019;s usability.</p></sec><sec sec-type="methods"><title>Methods</title><p>Between November 2023 and April 2025, a multidisciplinary research team developed a prototype and evaluated it during 2 rounds of pilot-testing. Participants were aged 10 to 15 years and attended local middle schools. Physical activity was assessed using a medical-grade, hip-worn accelerometer (ActiGraph wGT3X-BT). Acceptability was assessed using a validated questionnaire (the System Usability Scale) that has a maximum score of 100. To collect feedback for prototype improvements, semistructured interviews were conducted after each round of pilot-testing.</p></sec><sec sec-type="results"><title>Results</title><p>In the first round of pilot-testing, students (n=22) were active for 46.0% (6.9, SD 2.7 minutes) of the headset session, which lasted 15 (SD 0) minutes. After improving the prototype using feedback from the first round, students in the second round (n=10) were active for 5.8 (SD 3.1) minutes (62.4%) of the web-based assignment, which lasted 9.3 (SD 2.41) minutes, while still reporting &#x201C;good&#x201D; acceptability scores (mean 73.8, SD 17.2). There were no significant differences in acceptability ratings between the 2 pilot-testing rounds (<italic>P</italic>=.16), nor were there differences between boys and girls in round 1 (<italic>P</italic>=.79) or round 2 (<italic>P</italic>=.61).</p></sec><sec sec-type="conclusions"><title>Conclusions</title><p>The results of this iterative study indicate that mixed reality can be used to elicit physical activity in a classroom setting, at least for short assignments. However, further research is needed to determine longer-term use and effectiveness.</p></sec></abstract><kwd-group><kwd>digital learning tools</kwd><kwd>middle school</kwd><kwd>mixed reality</kwd><kwd>school-based physical activity</kwd><kwd>active learning technologies</kwd></kwd-group></article-meta></front><body><sec id="s1" sec-type="intro"><title>Introduction</title><p>Students in the United States spend close to 7 hours each weekday in school [<xref ref-type="bibr" rid="ref1">1</xref>], and for most of that time, they are sedentary [<xref ref-type="bibr" rid="ref2">2</xref>]. When students transition from elementary to secondary school, academic pressures increase, and there tends to be an increase in sedentary activity [<xref ref-type="bibr" rid="ref3">3</xref>]. In total, 89% of middle school students report being physically active for less than the recommended 60 minutes per day [<xref ref-type="bibr" rid="ref4">4</xref>]. While recent educational policy is aimed at increasing students&#x2019; academic performance [<xref ref-type="bibr" rid="ref5">5</xref>,<xref ref-type="bibr" rid="ref6">6</xref>], the policies have unintentionally led to decreased opportunities for physical activity [<xref ref-type="bibr" rid="ref7">7</xref>] and increased use of sedentary digital learning tools [<xref ref-type="bibr" rid="ref8">8</xref>].</p><p>The use of digital learning tools (ie, tablets, laptop computers, and smart boards) in school settings is not a new concept [<xref ref-type="bibr" rid="ref9">9</xref>], but the COVID-19 pandemic catalyzed rapid adoption [<xref ref-type="bibr" rid="ref10">10</xref>] as many schools switched to remote learning, contributing to significant increases in screen time for children [<xref ref-type="bibr" rid="ref11">11</xref>,<xref ref-type="bibr" rid="ref12">12</xref>]. Upon returning to in-person learning, the incorporation of technology into the curriculum became pervasive [<xref ref-type="bibr" rid="ref13">13</xref>,<xref ref-type="bibr" rid="ref14">14</xref>], further challenging educators and parents to find ways to decrease sedentary behaviors. Previous school-based interventions have focused on strategies such as physical activity breaks, active lessons, teacher training, school policy to limit screen time, and classroom modifications to encourage movement [<xref ref-type="bibr" rid="ref15">15</xref>]. Most of these interventions focus on moderate to vigorous physical activity yet neglect light physical activity, a lower intensity of movement that is related to positive health [<xref ref-type="bibr" rid="ref16">16</xref>,<xref ref-type="bibr" rid="ref17">17</xref>] and academic outcomes [<xref ref-type="bibr" rid="ref17">17</xref>,<xref ref-type="bibr" rid="ref18">18</xref>] and may be more feasible within the space limitations of an indoor classroom. Additionally, many school-based physical activity interventions avoid technology altogether. The development and deployment of new technology, such as mixed reality, could serve as a novel approach to leverage technology as a tool to increase physical activity in the school classroom.</p><p>Mixed reality devices, most commonly headsets, are equipped with cameras that allow users to view the real world around them. This allows them to operate headsets safely without fear of collision, which was a common problem with virtual reality headsets. Furthermore, these headsets now support technology that allows for identification and interpretation of gross motor movements of the arms, hands, and head, allowing for controller-free, direct interaction between user and application [<xref ref-type="bibr" rid="ref19">19</xref>]. A recent systematic review reported that there is preliminary evidence suggesting that mixed reality headsets could be used to increase physical activity through exergaming [<xref ref-type="bibr" rid="ref20">20</xref>]. More specifically, 2 popular virtual reality exergames, Gorilla Tag and Beat Saber, have been shown to increase light to moderate physical activity in early adolescents [<xref ref-type="bibr" rid="ref21">21</xref>]. Some researchers have started investigating the use of mixed reality headsets in physical education classes [<xref ref-type="bibr" rid="ref22">22</xref>], but it is currently unknown whether mixed reality headsets could be incorporated into traditional school curriculums, replacing sedentary computer time while encouraging more active learning environments.</p><p>The purpose of this study was to design a mixed reality prototype using an iterative process to optimize physical activity and satisfaction. The population of interest was middle school students, and the prototype was designed to be integrated into typical online academic assignments (ie, reading comprehension and vocabulary). The prototype program required students to punch, jump, and squat to complete the online academic tasks. The primary aim of this study was to determine how active students were when using the mixed reality technology to complete online academic assignments. The secondary aim was to examine the students&#x2019; acceptability of the mixed reality prototype.</p></sec><sec id="s2" sec-type="methods"><title>Methods</title><sec id="s2-1"><title>Study Design and Setting</title><p>This iterative study consisted of four phases: (1) prototype design and personnel training, (2) round 1 pilot-testing, (3) prototype modifications, and (4) round 2 pilot-testing. A detailed timeline can be found in <xref ref-type="fig" rid="figure1">Figure 1</xref>. The study was led by a multidisciplinary team trained in computer science, kinesiology, and developmental psychology. Prior to data collection, the research team determined a priori the study end points (metrics of success) that would inform the number of iterative testing and modifications. Specifically, the prototype would be considered complete once the product (1) engaged users in physical activity (ranging from light to moderate to vigorous) for at least 50% of the time spent completing an online academic assignment on average and (2) achieved an average score of at least 60 on the System Usability Scale (SUS) [<xref ref-type="bibr" rid="ref23">23</xref>,<xref ref-type="bibr" rid="ref24">24</xref>]. While developing the prototype, the research team had several discussions regarding the movements that the prototype would use, with the limitations of the Meta Quest 3 (Reality Labs) in mind. The team aimed to encourage movement of the upper and lower body while being mindful to minimize repetitive movements as they could cause injury. The Meta Quest 3 does not support feet tracking, but it does support hand and head tracking. On the basis of these constraints, the research team determined that punching would be used to elicit upper-body movements, enabled via hand tracking, and squatting and jumping would elicit lower-body movements, enabled via head tracking.</p><p>From November 2023 to November 2024, members of the research team designed a mixed reality prototype program that, when paired with a Meta Quest 3 headset, would allow students to use their body as a &#x201C;mouse&#x201D; to complete an online academic assignment. Personnel training took place in November 2024. During this time, 4 middle school students used the prototype to allow the research team to refine the study protocol and prototype program. Informal conversations with the students indicated that the prototype was enjoyable and worked as expected but could benefit from the addition of audio cues. This feedback was incorporated into the prototype prior to pilot-testing. Round 1 of pilot-testing took place in February 2025 at a local middle school. On the basis of the results of round 1, minor modifications were made to the prototype in March 2025. In April 2025, round 2 of pilot-testing took place at a laboratory space on the Louisiana State University campus.</p><fig position="float" id="figure1"><label>Figure 1.</label><caption><p>Iterative pilot study timeline of participant recruitment, prototype features, and modifications.</p></caption><graphic alt-version="no" mimetype="image" position="float" xlink:type="simple" xlink:href="formative_v10i1e83556_fig01.png"/></fig></sec><sec id="s2-2"><title>Participants and Procedures</title><p>To be eligible to participate in the pilot-testing of the prototype, students had to be aged between 10 and 15 years. Students were excluded if they did not have parental consent to participate.</p><p>The first round of testing took place at a local school that serves as a center for educational innovation and research. Prior to recruiting any participants, members of the research team met with the school administrators to provide them with details of the study and a demonstration of the prototype. After this initial meeting, the principal spoke with the sixth-grade teachers and determined that a math class would be the best option to participate in the study. Consent forms were sent home to parents. Those who agreed to allow their children to participate were asked to return a signed copy of the consent form to the school. Members of the research team collected consent forms from the school and then worked with the principal and math teacher to schedule a date and time to conduct the test. A total of 22 students returned signed consent forms and were eligible to participate in round 1 of pilot-testing.</p><p>The second round of testing took place when many of the local schools were on spring break. In an effort to not retest the same participants, local middle school students were recruited via word of mouth and flyers. In total, 10 parents returned signed consent forms, and their children were eligible to participate in round 2 of pilot-testing. After receiving signed consent forms, research team members worked with parents to schedule a testing day, which took place at a university laboratory space.</p><p>During both rounds of pilot-testing, members of the research team explained to the students, in an age-appropriate manner, what participation in the study would entail. If students were interested in participating in the study, they were asked to sign an assent form. Students were then provided with a short video tutorial created by the research team that introduced the assignments they would be working on and instructed them how to scroll and click. Members of the research team asked a few questions to ensure that the students knew how to complete the proper movements. A demonstration was provided if needed. Research team members then fitted the students with accelerometers and a Meta Quest 3 headset. Research team members recorded start and stop times. Additionally, the research team kept note of any exceptional factors that could impact testing (ie, motion sickness or ill-fitting headsets).</p></sec><sec id="s2-3"><title>Prototype Program</title><p>A modified virtual reality web browser was used for the online environment during pilot-testing. Upon putting on the Meta Quest 3 headset, students were presented with a large web page (<xref ref-type="fig" rid="figure2">Figure 2</xref>) with a yellow button to the left of the screen and a red button to the right. To begin the assignment, students had to press the yellow button, which set their default height. The default height value was used to detect when students jumped or squatted. Once the default height was established, participants could scroll down by jumping and scroll up by squatting. Clicking was a multistep process. Students first aimed with their left hand, which would move a clicker over the screen (<xref ref-type="fig" rid="figure3">Figure 3A</xref>). Once the clicker was hovering over the link or button, the participant would make a pinching motion (index finger and thumb closing) to lock the clicker in place. If the student accidentally placed the clicker in the wrong spot, they could press the red button on the right side of the screen to reset it. Once the clicker was locked, a large target appeared (<xref ref-type="fig" rid="figure3">Figure 3B</xref>); participants were encouraged to punch the target 3 times to finalize the click. Students were instructed to make hard, fast punches. However, interactions were triggered through collision, not speed. Thus, students could trigger the click with slower punches. Despite these activity-focused interactions, the virtual reality browser otherwise functioned as a normal web browser. It could display arbitrary web content, but for the purpose of this study, the browser was restricted to only showing the standardized academic assignments and the SUS.</p><fig position="float" id="figure2"><label>Figure 2.</label><caption><p>Orientation screen that students viewed when they initially put on the Meta Quest headset, including instructions on how to click, set their default height, and reset the clicker position.</p></caption><graphic alt-version="no" mimetype="image" position="float" xlink:type="simple" xlink:href="formative_v10i1e83556_fig02.png"/></fig><fig position="float" id="figure3"><label>Figure 3.</label><caption><p>Visual representation of clicking procedures: (1) locating the clicker, (2) making a pinching motion with the thumb and index fingers, and (3) punching the target 3 times. (A) Process to lock in the clicker and (B) process to finalize the click.</p></caption><graphic alt-version="no" mimetype="image" position="float" xlink:type="simple" xlink:href="formative_v10i1e83556_fig03.png"/></fig></sec><sec id="s2-4"><title>Academic Assignments</title><p>During both rounds, students were required to complete academic assignments. For round 1, they completed a vocabulary flash card exercise matching 30 terms to their meaning. If terms were connected to the incorrect meaning, students were required to try again. In addition to the vocabulary assignment, students completed 5 reading comprehension prompts, answering questions related to each prompt. In round 2, they completed the same 2 exercises, but the vocabulary exercise was changed from a flash card format, which did not require scrolling, to a web questionnaire format, which did require scrolling. The updated vocabulary quiz only consisted of 15 questions and did not require students to correct mistakes. This change was made to encourage more physical activity and de-emphasize the notion that students needed to select a &#x201C;correct&#x201D; answer, which was not the purpose of this iterative pilot study. In both rounds, the order of these 2 exercises was randomized across participants.</p></sec><sec id="s2-5"><title>Measures</title><sec id="s2-5-1"><title>Physical Activity</title><p>Physical activity was assessed using an ActiGraph wGT3X-BT accelerometer (Ametris) worn on a manufacturer-provided band and placed on the right hip in line with the midaxillary line. Research team members helped students attach the accelerometer before being fitted with the headset. Accelerometry wear time was adjusted using recorded headset start and stop times. Accelerometry data were downloaded and analyzed in ActiLife 6 (version 6.15.0; Ametris). Evenson cut points [<xref ref-type="bibr" rid="ref25">25</xref>], which are recommended for this age group [<xref ref-type="bibr" rid="ref26">26</xref>], in 15-second epochs were used to categorize physical activity into light, moderate, and vigorous. Percentages of time spent in total physical activity (light, moderate, and vigorous) were used as the outcome variable.</p></sec><sec id="s2-5-2"><title>Acceptability</title><p>The SUS [<xref ref-type="bibr" rid="ref23">23</xref>,<xref ref-type="bibr" rid="ref24">24</xref>], which is commonly used to judge the user-friendliness of software systems, was used to measure acceptability. Students answered the 11-item survey in the modified virtual reality web browser. Questions 1 through 10 used a 5-point Likert scale ranging from 1 (&#x201C;strongly disagree&#x201D;) to 5 (&#x201C;strongly agree&#x201D;). Raw scores are converted to a score of 0 to 100. Lower scores indicated lower usability: scores from 0 to 49.9 correspond to &#x201C;not acceptable,&#x201D; scores from 50 to 69.9 correspond to &#x201C;marginally acceptable,&#x201D; and scores from 70 to 100 correspond to &#x201C;acceptable.&#x201D; In 2009 [<xref ref-type="bibr" rid="ref24">24</xref>], an adjective rating scale was added to the SUS (question 11). This 11th question aimed to better understand the overall user-friendliness of the prototype using a Likert scale ranging from 1 ( &#x201C;worst imaginable&#x201D;) to 7 (&#x201C;best imaginable&#x201D;). The 11th item is interpreted on its own and is not included in the total SUS score. Overall, the instrument demonstrates excellent reliability (Cronbach &#x03B1;=0.91) and a high level of concurrent validity (<italic>r</italic>=0.81) [<xref ref-type="bibr" rid="ref27">27</xref>].</p></sec><sec id="s2-5-3"><title>Prototype Program</title><p>To increase data integrity, data on device use were automatically recorded by the virtual web browser. Specifically, start times, end times, responses to the standardized assignments, SUS scores, and user identifiers were automatically recorded and stored in a remote database. Deidentified assigned nicknames were used to cross-reference physical activity data with performance data.</p></sec><sec id="s2-5-4"><title>Semistructured Interview</title><p>Following completion of their session, students were asked multiple questions that probed their opinion on the experience with the headset program, what they liked most about the experience, and suggestions for improvement.</p></sec></sec><sec id="s2-6"><title>Statistical Analysis</title><p>The statistical software SPSS (version 29; IBM Corp) was used. Descriptive statistics were calculated for all variables. Mann-Whitney <italic>U</italic> tests were used to identify differences in SUS scores (ie, boys vs girls and rounds 1 vs 2). The &#x03B1; level was set at .05 a priori.</p></sec><sec id="s2-7"><title>Ethical Considerations</title><p>This study was approved by the institutional review boards of the Pennington Biomedical Research Center (2024-052; approved on October 3, 2024) and Louisiana State University (IRBAM-24-1094; approved on December 5, 2024) and conformed to the latest Declaration of Helsinki. Written parental consent and child assent were obtained before data collection. To maintain privacy and confidentiality, students were assigned a study-specific identification number and a randomly generated code to connect physical activity data with headset use. Students were provided with small tokens of appreciation (ie, notebooks, small fidget toys, stickers, and lip balm) and snacks (ie, fruit, water, granola bars, and fruit snacks) as compensation for their participation.</p></sec></sec><sec id="s3" sec-type="results"><title>Results</title><sec id="s3-1"><title>Overview</title><p>Results from round 1 and 2 of pilot-testing are presented separately to highlight the changes that occurred during this iterative development process. Demographic characteristics of users in each round are reported in <xref ref-type="table" rid="table1">Table 1</xref>.</p><table-wrap id="t1" position="float"><label>Table 1.</label><caption><p>Characteristics of round 1 and 2 of pilot-testing for the mixed reality prototype.</p></caption><table id="table1" frame="hsides" rules="groups"><thead><tr><td align="left" valign="bottom">Variable</td><td align="left" valign="bottom">Values</td></tr></thead><tbody><tr><td align="left" valign="top" colspan="2">Round 1 (n=22)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Age (years), mean (SD)</td><td align="left" valign="top">12.3 (0.4)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Height (cm), mean (SD)</td><td align="left" valign="top">155.3 (7.6)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Weight (kg), mean (SD)</td><td align="left" valign="top">49.9 (14.5)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>BMI (kg/m<sup>2</sup>), mean (SD)</td><td align="left" valign="top">20.2 (4.2)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Healthy weight, n (%)</td><td align="left" valign="top">15 (68.2)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Boys, n (%)</td><td align="left" valign="top">15 (68.2)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>White, n (%)</td><td align="left" valign="top">14 (63.6)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Hispanic origin, n (%)</td><td align="left" valign="top">3 (13.6)</td></tr><tr><td align="left" valign="top" colspan="2">Round 2 (n=10)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Age (years), mean (SD)</td><td align="left" valign="top">11.8 (0.6)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Height (cm), mean (SD)</td><td align="left" valign="top">149.4 (6.5)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>BMI (kg/m<sup>2</sup>), mean (SD)</td><td align="left" valign="top">18.6 (3.3)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Healthy weight, n (%)</td><td align="left" valign="top">7 (70.0)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Weight (kg), mean (SD)</td><td align="left" valign="top">41.9 (9.8)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Boys, n (%)</td><td align="left" valign="top">6 (60.0)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>White, n (%)</td><td align="left" valign="top">8 (80.0)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Hispanic origin, n (%)</td><td align="left" valign="top">0 (0.0)</td></tr></tbody></table></table-wrap></sec><sec id="s3-2"><title>Round 1</title><p>A total of 22 middle school students (mean age 12.3, SD 0.4 years; n=15, 68.2% boys) participated in round 1 of pilot-testing. Students spent 6.9 (SD 2.7) minutes (46.0%) of the session, which lasted a mean 15 (SD 0) minutes, engaging in physical activity, as shown in <xref ref-type="table" rid="table2">Table 2</xref>. The average acceptability score was 80.7 (SD 16.1), which was in the highest range for acceptability. <xref ref-type="fig" rid="figure4">Figure 4</xref> [<xref ref-type="bibr" rid="ref23">23</xref>,<xref ref-type="bibr" rid="ref24">24</xref>], used with permission, shows how SUS scores are usually interpreted. The average acceptability score of 80.7 lies in the &#x201C;excellent&#x201D; range. There were no significant differences (<italic>P</italic>=.79) in acceptability scores between boys (mean 83.17, SD 11.47) and girls (mean 74.58, SD 24.67).</p><table-wrap id="t2" position="float"><label>Table 2.</label><caption><p>Physical activity measured through accelerometry during rounds 1 and 2 of pilot-testing for the mixed reality prototype.</p></caption><table id="table2" frame="hsides" rules="groups"><thead><tr><td align="left" valign="top">Variable</td><td align="left" valign="top">Mean (SD)</td></tr></thead><tbody><tr><td align="left" valign="top" colspan="2">Round 1</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Sedentary time (min)</td><td align="char" char="." valign="top">8.1 (2.2)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Light physical activity (min)</td><td align="char" char="." valign="top">4.7 (1.2)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Moderate physical activity (min)</td><td align="char" char="." valign="top">1.1 (0.6)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Vigorous physical activity (min)</td><td align="char" char="." valign="top">1.1 (0.9)</td></tr><tr><td align="left" valign="top" colspan="2">Round 2</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Sedentary time (min)</td><td align="char" char="." valign="top">3.5 (2.0)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Light physical activity (min)</td><td align="char" char="." valign="top">4.2 (2.4)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Moderate physical activity (min)</td><td align="char" char="." valign="top">1.1 (0.3)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Vigorous physical activity (min)</td><td align="char" char="." valign="top">0.5 (0.4)</td></tr></tbody></table></table-wrap><fig position="float" id="figure4"><label>Figure 4.</label><caption><p>System Usability Scale scores from rounds 1 and 2 of pilot-testing, including acceptability ranges, adjective ratings, and mean and SD of acceptability scores (modified figure used with permission [<xref ref-type="bibr" rid="ref23">23</xref>,<xref ref-type="bibr" rid="ref24">24</xref>]).</p></caption><graphic alt-version="no" mimetype="image" position="float" xlink:type="simple" xlink:href="formative_v10i1e83556_fig04.png"/></fig><p>Semistructured interviews and direct observation revealed several key insights. As suggested by the high SUS score, students indicated that they would be willing to use the prototype to complete school assignments both at home and at school. Students enjoyed most of the features (eg, punching, jumping, sound effects, and ease of use), but some design aspects could be improved. For example, the cursor (pinching and aiming) required precise aim to use; even a small movement could cause a student to miss a button or link. Students suggested making a larger pointer or expanding the selection area to fix this issue. Proctors noticed that some students, instead of punching the target, slapped the target 3 times by making a small movement with their hand.</p></sec><sec id="s3-3"><title>Round 2</title><p>Following round 1 of pilot-testing, the research team made several adjustments to the prototype to increase physical activity and improve usability. First, the mechanism to scroll was modified to encourage more movement to meet the physical activity goal. In round 1, participants held a squat to scroll the page up. This was modified in round 2 so that, for every squat, the modified web browser would only scroll up a fixed distance, thereby requiring participants to squat repeatedly to scroll up. Second, to require full arm movement, the software was modified to require students&#x2019; hands to return to their body after punching before their next punch. Finally, an internal clock was added to allow researchers to precisely analyze physical activity data. In addition to these modifications, researchers changed the vocabulary flash cards to a vocabulary quiz that would require students to scroll the page more. The reading comprehension questions did not change in round 2.</p><p>Round 2 participants comprised 10 local middle school students (mean 11.82, SD 0.63 years; n=6, 60% boys). Students were physically active for 5.8 (SD 3.1) minutes (62.4%) of the session, which lasted a mean 9.3 (SD 2.41) minutes (<xref ref-type="table" rid="table2">Table 2</xref>). The average acceptability score for round 2 was 73.8 (SD 17.2; <xref ref-type="fig" rid="figure4">Figure 4</xref>), which remained in the highest category for acceptability and was not statistically significantly different from that in round 1 (<italic>P</italic>=.16). There was no significant difference (<italic>P</italic>=.61) in round 2 acceptability scores between boys (mean 78.75, SD 8.48) and girls (mean 66.25, SD 25.37). Nearly half (4/10, 40%) of the participants reported the user-friendliness of the prototype program as &#x201C;best imaginable,&#x201D; which was the highest score. Similarly to round 1 of pilot-testing, the students stated during the semistructured interview that they would use the prototype to complete school assignments. However, a few students stated that, due to the weight of the headset, they would only use the prototype to complete short (15-20 minutes) assignments. Jumping and punching remained the favorite features of both boys and girls. Additionally, the issues with aiming the cursor remained a common complaint.</p></sec></sec><sec id="s4" sec-type="discussion"><title>Discussion</title><sec id="s4-1"><title>Principal Findings</title><p>This iterative study resulted in an acceptable mixed reality prototype program that engaged students in physical activity while they completed online academic assignments. To our knowledge, this is the first study to test mixed reality technology as a method for increasing physical activity while completing schoolwork. During round 2 of pilot-testing, students were physically active for most of the time it took them to complete the online assignments, and the students also provided high scores for user satisfaction. Semistructured interviews with students further emphasized that the mixed reality prototype program was a digital learning tool that they would be willing to use at school and at home. Of note, teachers and school administrators were also interested in deploying the mixed reality prototype program in their classes. By conducting 2 rounds of testing with minor modification in between, researchers were able to demonstrate that this prototype can engage students in physical activity 46% to 62.4% of the time it takes to complete online assignments. This ultimately equates to approximately 6 to 7 minutes of physical activity in a 10- to 15-minute academic task. Overall, the results of this study are congruent with those of popular virtual reality exergames such as Beat Saber or Gorilla Tag [<xref ref-type="bibr" rid="ref21">21</xref>].</p></sec><sec id="s4-2"><title>Comparison With Prior Work</title><p>Traditionally, school-based physical activity interventions have been designed to incorporate moderate to vigorous physical activity into classroom settings. These methods often result in modest changes in moderate to vigorous physical activity during intervention lessons [<xref ref-type="bibr" rid="ref28">28</xref>]. Unfortunately, teachers and students might be hesitant to engage in moderate to vigorous physical activity in classroom settings. Teachers often have a limited time to incorporate physical activity into lessons [<xref ref-type="bibr" rid="ref29">29</xref>], and the classroom size might make it difficult to engage in higher-intensity physical activity. Qualitative data indicate that students are hesitant to engage in higher-intensity physical activity because they do not want to sweat or change clothes [<xref ref-type="bibr" rid="ref30">30</xref>], and teachers are reluctant due to limited space [<xref ref-type="bibr" rid="ref31">31</xref>] and competing priorities [<xref ref-type="bibr" rid="ref32">32</xref>].</p><p>Instead, replacing sedentary computer time that is already part of classroom instruction with physical activity computer time may be a less disruptive and more feasible strategy to increase minutes of physical activity. In this study, the developed prototype program elicited similar physical activity responses to those elicited by traditional interventions [<xref ref-type="bibr" rid="ref28">28</xref>], all while working with a digital learning tool that can be easily incorporated into the learning environment.</p><p>These results contribute promising evidence to the nascent research that suggests that mixed reality technology can be used to increase physical activity [<xref ref-type="bibr" rid="ref20">20</xref>]. Most school-based trials to date that have tested mixed reality, augmented reality, or virtual reality have focused on incorporating activity-promoting technology into physical education classes [<xref ref-type="bibr" rid="ref22">22</xref>], whereas this prototype was designed to be integrated into a traditional academic subject matter classroom with academic assignments such as reading comprehension. One mixed reality trial integrated into physical education focused on improving children&#x2019;s motivation to be active and their motor skills and physical fitness but did not evaluate minutes spent engaged in physical activity [<xref ref-type="bibr" rid="ref33">33</xref>]. A trial that did focus on increasing physical activity was conducted by Ahn et al [<xref ref-type="bibr" rid="ref34">34</xref>] and involved a virtual fitness buddy ecosystem that would allow children aged 6 to 11 years to interact with a virtual dog to set and meet self-determined physical activity goals. Results indicated that the ecosystem was effective at significantly increasing light physical activity and decreasing sedentary behavior during an after-school program [<xref ref-type="bibr" rid="ref34">34</xref>]. Similarly to the work by Ahn et al [<xref ref-type="bibr" rid="ref34">34</xref>], this study found that using a mixed reality prototype primarily increased light physical activity. The integration of movement, even light physical activity, has been shown to benefit physical health and academic outcomes [<xref ref-type="bibr" rid="ref16">16</xref>-<xref ref-type="bibr" rid="ref18">18</xref>].</p><p>The findings of this study indicate that mixed reality technology might encourage more physical activity during a traditionally sedentary school day. The broader evidence base for exergaming and active video games is mixed. Many have found that these methods are effective at promoting physical activity and decreasing sedentary behavior [<xref ref-type="bibr" rid="ref20">20</xref>,<xref ref-type="bibr" rid="ref33">33</xref>-<xref ref-type="bibr" rid="ref35">35</xref>]. Furthermore, there is evidence suggesting that exergaming and active video games might improve sleep hygiene and health-related quality of life [<xref ref-type="bibr" rid="ref36">36</xref>]. However, one small trial of 49 children found significant increases in sedentary behavior but no significant increases in other screen time [<xref ref-type="bibr" rid="ref37">37</xref>]. The overall quality of evidence in active video game research is low, and researchers should aim for increasing methodological rigor and more balanced reporting of findings [<xref ref-type="bibr" rid="ref38">38</xref>].</p></sec><sec id="s4-3"><title>Limitations</title><p>This study was designed to examine physical activity while wearing the headset; thus, the difference between typical student classroom activity and activity using our prototype is not known. Although typical in-class assignments are generally sedentary, future trials should examine students&#x2019; physical activity levels with and without the prototype, such as comparing physical activity while using a traditional computer vs the mixed reality headset. Additionally, researchers should examine whether integration of the prototype contributes to a meaningful increase in physical activity throughout the school day and whether the effect can be sustained over weeks and months. The assignment time was set for 15 minutes. However, with the addition of an internal clock in round 2, it was evident that students completed the assignment in approximately 9 minutes, and some indicated that the weight of the headset precluded longer sessions. The difference in assignment completion time is most likely attributed to the change in the academic assignment from 30 vocabulary flash cards to a 15-item vocabulary quiz. Future studies should examine the duration of time that students will tolerate wearing the headset while balancing engagement in physical activity and acceptability. Finally, future research should examine acceptability from the classroom teachers and school administrators to ensure that the prototype is helpful and not disruptive to classroom instruction. Anecdotal information from the pilot test indicated that the school administrators were positive about the prototype and the math and science teachers were interested in integrating the mixed reality headset into the classroom, but this should be explored in a structured interview in future trials.</p></sec><sec id="s4-4"><title>Conclusions</title><p>The continued use of digital learning tools in educational settings has led to increases in screen time and sedentary behavior [<xref ref-type="bibr" rid="ref7">7</xref>,<xref ref-type="bibr" rid="ref8">8</xref>,<xref ref-type="bibr" rid="ref11">11</xref>]. These trends have challenged educators to identify new ways to incorporate movement into classroom settings. The results of this iterative study indicate that this mixed reality prototype program might be a pathway to increase physical activity in the classroom in a way that is acceptable and enjoyed by students. Future research is needed to determine longer-term usability and effectiveness.</p></sec></sec></body><back><notes><sec><title>Funding</title><p>This project was funded by the Louisiana Blue Foundation. KES is supported by the National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health (NIH) under award T32DK064584. The research reported in this publication was supported by Pennington Biomedical Research Center grant U54 GM104940 funded by the NIH National Institute of General Medical Sciences through the Institutional Development Award Program Infrastructure for Clinical and Translational Research, as well as by a Nutrition Obesity Research Center grant (P30DK072476) titled &#x201C;Nutrition and Metabolic Health Through the Lifespan&#x201D; sponsored by the NIH National Institute of Diabetes and Digestive and Kidney Diseases. This content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.</p></sec><sec><title>Disclaimer</title><p>Generative artificial intelligence was not used in any portion of manuscript generation.</p></sec><sec><title>Data Availability</title><p>The datasets generated or analyzed during this study are not publicly available due to the formative nature of the data and the small sample size but are available from the corresponding author on reasonable request.</p></sec></notes><fn-group><fn fn-type="con"><p>Conceptualization: AES, DCS</p><p>Data curation: AES, CWF, DCS, KES, MPP, PN, SC</p><p>Formal analysis: KES</p><p>Investigation: AES, CWF, DCS, KES, MPP, PN, SC</p><p>Methodology: AES, DCS, SC</p><p>Project administration: KES, PN</p><p>Software: CWF, DCS, MPP</p><p>Supervision: AES, DCS, SC</p><p>Visualization: CWF, KES</p><p>Writing&#x2014;original draft: CWF, KES</p><p>Writing&#x2014;review and editing: AES, CWF, DCS, KES, 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