<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD Journal Publishing DTD v2.0 20040830//EN" "journalpublishing.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="2.0" xml:lang="en" article-type="letter"><front><journal-meta><journal-id journal-id-type="nlm-ta">JMIR Form Res</journal-id><journal-id journal-id-type="publisher-id">formative</journal-id><journal-id journal-id-type="index">27</journal-id><journal-title>JMIR Formative Research</journal-title><abbrev-journal-title>JMIR Form Res</abbrev-journal-title><issn pub-type="epub">2561-326X</issn><publisher><publisher-name>JMIR Publications</publisher-name><publisher-loc>Toronto, Canada</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">v9i1e73394</article-id><article-id pub-id-type="doi">10.2196/73394</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Letter</subject></subj-group></article-categories><title-group><article-title>Examining the Relationship Between Assertiveness and Anxiety in First- and Second-Year US Medical Students</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes" equal-contrib="yes"><name name-style="western"><surname>Shaw</surname><given-names>Jonathan</given-names></name><degrees>BS</degrees><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="fn" rid="equal-contrib1">*</xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name name-style="western"><surname>Hagerty</surname><given-names>James</given-names></name><degrees>BS</degrees><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="fn" rid="equal-contrib1">*</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Masada</surname><given-names>Kristen</given-names></name><degrees>BA</degrees><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Won</surname><given-names>Angelene Eunji</given-names></name><degrees>BA</degrees><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Lai</surname><given-names>Ashley</given-names></name><degrees>BS</degrees><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Shin</surname><given-names>Jisu</given-names></name><degrees>BS</degrees><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Le</surname><given-names>Van</given-names></name><degrees>BA</degrees><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Phung</surname><given-names>Brenton</given-names></name><degrees>BA</degrees><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Lai</surname><given-names>Charles</given-names></name><degrees>BS</degrees><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Bota</surname><given-names>Peter</given-names></name><degrees>BA</degrees><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Jacobs</surname><given-names>Aaron</given-names></name><degrees>PhD</degrees><xref ref-type="aff" rid="aff2">2</xref></contrib></contrib-group><aff id="aff1"><institution>School of Medicine, California University of Science and Medicine</institution><addr-line>1501 Violet St</addr-line><addr-line>Colton</addr-line><country>United States</country></aff><aff id="aff2"><institution>Medical Education, California University of Science and Medicine</institution><country>United States</country></aff><contrib-group><contrib contrib-type="editor"><name name-style="western"><surname>Sarvestan</surname><given-names>Javad</given-names></name></contrib></contrib-group><contrib-group><contrib contrib-type="reviewer"><name name-style="western"><surname>Andricioaei</surname><given-names>Anton</given-names></name></contrib><contrib contrib-type="reviewer"><name name-style="western"><surname>Kotyk</surname><given-names>Taras</given-names></name></contrib></contrib-group><author-notes><corresp>Correspondence to Jonathan Shaw, BS, School of Medicine, California University of Science and Medicine, 1501 Violet St, Colton, 92324, United States, 1 9094980036; <email>jonathan.shaw@md.cusm.edu</email></corresp><fn fn-type="equal" id="equal-contrib1"><label>*</label><p>these authors contributed equally</p></fn></author-notes><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>20</day><month>6</month><year>2025</year></pub-date><volume>9</volume><elocation-id>e73394</elocation-id><history><date date-type="received"><day>03</day><month>03</month><year>2025</year></date><date date-type="rev-recd"><day>27</day><month>05</month><year>2025</year></date><date date-type="accepted"><day>29</day><month>05</month><year>2025</year></date></history><copyright-statement>&#x00A9; Jonathan Shaw, James Hagerty, Kristen Masada, Angelene Eunji Won, Ashley Lai, Jisu Shin, Van Le, Brenton Phung, Charles Lai, Peter Bota, Aaron Jacobs. Originally published in JMIR Formative Research (<ext-link ext-link-type="uri" xlink:href="https://formative.jmir.org">https://formative.jmir.org</ext-link>), 20.6.2025. </copyright-statement><copyright-year>2025</copyright-year><license license-type="open-access" xlink:href="https://creativecommons.org/licenses/by/4.0/"><p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">https://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in JMIR Formative Research, is properly cited. The complete bibliographic information, a link to the original publication on <ext-link ext-link-type="uri" xlink:href="https://formative.jmir.org">https://formative.jmir.org</ext-link>, as well as this copyright and license information must be included.</p></license><self-uri xlink:type="simple" xlink:href="https://formative.jmir.org/2025/1/e73394"/><abstract><p>In this single-institution remote survey study of a California medical school, anxiety and assertiveness were found to inversely correlate in preclinical medical students.</p></abstract><kwd-group><kwd>assertiveness</kwd><kwd>anxiety</kwd><kwd>medical student</kwd><kwd>preclinical</kwd><kwd>medical education</kwd><kwd>well-being</kwd></kwd-group></article-meta></front><body><sec id="s1" sec-type="intro"><title>Introduction</title><p>Confident and assertive physicians&#x2019; development is essential to effective patient care [<xref ref-type="bibr" rid="ref1">1</xref>]. Medical training shapes students&#x2019; knowledge, skills, and interpersonal abilities, including confidence and assertiveness, which evolve throughout education [<xref ref-type="bibr" rid="ref2">2</xref>]. Assertiveness influences clinical decision-making, patient communication, and professional success [<xref ref-type="bibr" rid="ref3">3</xref>]. Further, assertiveness has been shown to improve feelings of anxiety, stress, and depression [<xref ref-type="bibr" rid="ref4">4</xref>]. Although literature confirms that training can improve assertiveness, factors that affect assertiveness and its gender-based differences have been underexplored in medical students, who face uniquely stressful hierarchical environments [<xref ref-type="bibr" rid="ref1">1</xref>]. A better understanding of assertiveness and anxiety correlations can help educators tailor interventions to support students&#x2019; well-being and competence. To guide future curricular improvements, we investigate this relationship through the Simple Rathus Assertiveness Scale-Short Form (SRAS-SF) and the General Anxiety Disorder 7-item (GAD-7) assessment.</p></sec><sec id="s2" sec-type="methods"><title>Methods</title><sec id="s2-1"><title>Participants and Recruitment</title><p>First-year and second-year medical students (n=120 and n=126, respectively) from a California allopathic school with a pass/fail curriculum completed an anonymous Google Forms survey from January 31 to February 29, 2024 (invited via institutional email). Submission of multiple responses was prevented via Google Forms&#x2019; single-response function. For study inclusion, students had to be in their preclinical years of the school&#x2019;s Doctor of Medicine program; else, they were excluded. A convenience sample of 30 responses (23 and 7 responses from first-year and second-year medical students, respectively) was collected (<xref ref-type="table" rid="table1">Table 1</xref>).</p><table-wrap id="t1" position="float"><label>Table 1.</label><caption><p>Descriptive statistics (responses: N=30).</p></caption><table id="table1" frame="hsides" rules="groups"><thead><tr><td align="left" valign="top">Survey items</td><td align="left" valign="top">Value</td></tr></thead><tbody><tr><td align="left" valign="top" colspan="2">Demographics, n (%)</td></tr><tr><td align="left" valign="top" colspan="2"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Which school year are you?</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>First year</td><td align="left" valign="top">23 (77)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Second year</td><td align="left" valign="top">7 (23)</td></tr><tr><td align="left" valign="top" colspan="2"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>What gender do you identify as?</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Male</td><td align="left" valign="top">13 (43)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Female</td><td align="left" valign="top">17 (57)</td></tr><tr><td align="left" valign="top" colspan="2">SRAS-SF<sup><xref ref-type="table-fn" rid="table1fn1">a</xref></sup><sup>,</sup><sup><xref ref-type="table-fn" rid="table1fn2">b</xref></sup> score, median (IQR)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>When I am eating out and the food I am served is not cooked the way I like it, I complain to the person serving it</td><td align="left" valign="top">&#x2212;2.00 (&#x2212;2.25 to 1.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>There are times when I look for a good strong argument</td><td align="left" valign="top">1.00 (&#x2212;2.00 to 2.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>I try as hard in life to get ahead as most people like me do</td><td align="left" valign="top">1.00 (0.50 to 2.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>If a famous person were talking in a crowd and I thought he/she was wrong, I would get up and say what I thought</td><td align="left" valign="top">&#x2212;2.00 (&#x2212;3.00 to &#x2212;1.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>If someone has been telling false and bad stories about me, I see him or her as soon as possible to &#x201C;have a talk&#x201D; about it</td><td align="left" valign="top">1.00 (&#x2212;2.00 to 2.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>I complain about poor service when I am eating out or in other places</td><td align="left" valign="top">&#x2212;2.00 (&#x2212;2.00 to &#x2212;1.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>If a couple near me in the theater were talking rather loudly, I would ask them to be quite or to go somewhere else and talk</td><td align="left" valign="top">&#x2212;1.00 (&#x2212;2.25 to 1.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>I am quick to say what I think</td><td align="left" valign="top">0 (&#x2212;1.00 to 1.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Most people stand up for themselves more than I do</td><td align="left" valign="top">0 (&#x2212;2.00 to 2.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>At times I have not made or gone on dates because of my shyness</td><td align="left" valign="top">&#x2212;1.00 (&#x2212;2.25 to 1.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>If a person serving in a store has gone to a lot of trouble to show me something which I do not really like, I have a hard time saying, &#x201C;No.&#x201D;</td><td align="left" valign="top">&#x2212;1.00 (&#x2212;2.00 to 1.25)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>To be honest, people often get the better of me</td><td align="left" valign="top">&#x2212;1.00 (&#x2212;2.00 to 1.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>I do not like making phone calls to businesses or companies</td><td align="left" valign="top">1.50 (0.25 to 2.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>I feel silly if I return things I don&#x2019;t like to the store that I bought them from</td><td align="left" valign="top">&#x2212;1.50 (&#x2212;2.00 to 1.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>If a close relative that I like was upsetting me, I would hide my feelings rather than say that I was upset</td><td align="left" valign="top">&#x2212;1.00 (&#x2212;1.25 to 2.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>I have sometimes not asked questions for the fear of sounding stupid</td><td align="left" valign="top">2.00 (&#x2212;1.25 to 2.25)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>During an argument, I am sometimes afraid that I will get so upset that I will shake all over</td><td align="left" valign="top">&#x2212;2.00 (&#x2212;3.00 to &#x2212;0.50)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>I often have a hard time saying, &#x201C;No.&#x201D;</td><td align="left" valign="top">1.00 (&#x2212;2.00 to 2.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>When someone says I have done well, I sometimes just don&#x2019;t know what to say</td><td align="left" valign="top">1.00 (&#x2212;2.00 to 1.25)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>SRAS-SF total score</td><td align="left" valign="top">&#x2212;0.11 (&#x2212;0.76 to 0.84)</td></tr><tr><td align="left" valign="top" colspan="2">GAD-7<sup><xref ref-type="table-fn" rid="table1fn3">c</xref></sup><sup>,</sup><sup><xref ref-type="table-fn" rid="table1fn4">d</xref></sup> assessment score, median (IQR)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Feeling nervous, anxious, or on edge</td><td align="left" valign="top">1.00 (0.75 to 2)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Not being able to stop or control worrying</td><td align="left" valign="top">0 (0 to 1.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Worrying too much about different things</td><td align="left" valign="top">1.00 (0 to 2.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Trouble relaxing</td><td align="left" valign="top">1.00 (0 to 2.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Being so restless that it&#x2019;s hard to sit still</td><td align="left" valign="top">0 (0 to 1.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Becoming easily annoyed or irritable</td><td align="left" valign="top">1.00 (0 to 2.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>Feeling afraid as if something awful might happen</td><td align="left" valign="top">0.50 (0 to 1.00)</td></tr><tr><td align="left" valign="top"><named-content content-type="indent">&#x00A0;&#x00A0;&#x00A0;&#x00A0;</named-content>GAD-7 total score</td><td align="left" valign="top">4.50 (2.00 to 11.25)</td></tr></tbody></table><table-wrap-foot><fn id="table1fn1"><p><sup>a</sup>SRAS-SF: Simple Rathus Assertiveness Scale-Short Form.</p></fn><fn id="table1fn2"><p><sup>b</sup>SRAS-SF scoring: The SRAS-SF consists of 19 statements that participants indicate their agreement with, using a 6-point Likert-scale (&#x2212;3=&#x201C;very much unlike me&#x201D;; 3=&#x201C;very much like me&#x201D;). Responses are averaged, resulting in total scores between &#x2212;3 (less assertive) and 3 (more assertive).</p></fn><fn id="table1fn3"><p><sup>c</sup>GAD-7: General Anxiety Disorder 7-item.</p></fn><fn id="table1fn4"><p><sup>d</sup>GAD-7 scoring: The GAD-7 consists of 7 statements about anxiety symptoms, with participants indicating how often they experienced these symptoms within the last 2 weeks by using a 4-point Likert scale (0=&#x201C;not at all&#x201D;; 3=&#x201C;nearly every day&#x201D;). These scores are added together to determine anxiety severity: 0&#x2010;4 (minimal), 5&#x2010;9 (mild), 10&#x2010;14 (moderate), and 15&#x2010;21 (severe anxiety).</p></fn></table-wrap-foot></table-wrap></sec><sec id="s2-2"><title>Measures</title><p>The survey included demographic questions (school year and gender), 19 SRAS-SF items [<xref ref-type="bibr" rid="ref5">5</xref>], and the GAD-7. The SRAS-SF and GAD-7 were presented in separate, randomized sections.</p></sec><sec id="s2-3"><title>Statistical Analysis</title><p>IBM SPSS Statistics 28.0.1.0 (IBM Corp) was used for analysis. Due to the small sample size (n=30), a Shapiro-Wilk test was used to assess if data were normally distributed [<xref ref-type="bibr" rid="ref6">6</xref>]. Parametric (2-tailed independent samples <italic>t</italic> test and Pearson correlation) and nonparametric (Kruskal-Wallis test and Spearman correlation) statistical tests were used based on data distribution normality. Gender and school year were used as grouping variables.</p></sec><sec id="s2-4"><title>Ethical Considerations</title><p>This study received ethical approval from the California University of Science and Medicine Institutional Review Board (approval: HS-2024&#x2010;03) on January 22, 2024. Informed consent for primary data collection and secondary analyses of the data was obtained from all participants. Participants received no compensation for participation.</p></sec></sec><sec id="s3" sec-type="results"><title>Results</title><p>The Shapiro-Wilk test indicated that SRAS-SF scores (<italic>P</italic>=.07) were normally distributed, while GAD-7 scores (<italic>P</italic>=.01) and all individual survey items (<italic>P</italic>&#x003C;.05) were not normally distributed.</p><p>Per the Kruskal-Wallis test for examining differences between responses by school year and gender, first-year medical students were more likely to feel uncomfortable when returning purchases (<italic>P</italic>=.03), and female participants were more likely to ask loud theater couples to be quiet (<italic>P</italic>=.05). No differences in GAD-7 scores by school year (<italic>P</italic>=.67) or gender (<italic>P</italic>=.52) were noted.</p><p>As the overall SRAS-SF scores were normally distributed, an independent <italic>t</italic> test was used; it found no significant differences in SRAS-SF scores by school year (<italic>P</italic>=.95) or gender (<italic>P</italic>=.62).</p><p>A Pearson correlation revealed a strong negative correlation between SRAS-SF and GAD-7 scores (n=30, <italic>r</italic>=&#x2212;0.624; <italic>P</italic>&#x003C;.001; <xref ref-type="fig" rid="figure1">Figure 1</xref>).</p><fig position="float" id="figure1"><label>Figure 1.</label><caption><p>Scatterplot of GAD-7 scores by SRAS-SF scores. This figure was generated by using SPSS, and it visualizes the line of best fit between the GAD-7 and SRAS-SF scores. The <italic>R</italic><sup>2</sup> value is 0.39, and the <italic>r</italic> value is &#x2212;0.62, indicating a strong negative correlation between GAD-7 and SRAS-SF scores. GAD-7: General Anxiety Disorder 7-item; SRAS-SF: Simple Rathus Assertiveness Scale-Short Form.</p></caption><graphic alt-version="no" mimetype="image" position="float" xlink:type="simple" xlink:href="formative_v9i1e73394_fig01.png"/></fig></sec><sec id="s4" sec-type="discussion"><title>Discussion</title><sec id="s4-1"><title>Principal Findings</title><p>The differences between first-year and second-year medical students&#x2019; confidence toward returning purchases may reflect cohort-based personality variation. Female participants&#x2019; greater likelihood of addressing disruptive behavior may reflect gender differences in assertiveness, warranting further study on socialization and professional behaviors in medical education.</p><p>The strong negative correlation between assertiveness and anxiety aligns with research indicating medical students&#x2019; high anxiety levels [<xref ref-type="bibr" rid="ref7">7</xref>], raising the possibility that greater assertiveness is linked to lower anxiety, though causality cannot be inferred [<xref ref-type="bibr" rid="ref8">8</xref>]. To determine if assertiveness development mitigates anxiety, future studies should explore whether interventions targeting assertiveness influence students&#x2019; well-being. Given anxiety&#x2019;s impact on academic performance and mental health, tailored strategies could help students in their training.</p><p>This study focuses on preclinical students, limiting applicability to clinical training or residency students. However, medical students face increasing anxiety due to high-stakes evaluations and residency match competitiveness [<xref ref-type="bibr" rid="ref9">9</xref>,<xref ref-type="bibr" rid="ref10">10</xref>]. Larger longitudinal studies could clarify this relationship and better inform future interventions.</p></sec><sec id="s4-2"><title>Limitations</title><p>Our small, single-institution sample limits generalizability and statistical power. Future studies should include multiple institutions to account for educational and cultural variations. Additionally, this study focuses on preclinical students, limiting relevance to clinical training or residency students.</p></sec><sec id="s4-3"><title>Conclusions</title><p>Our findings support the existing literature and suggest that assertiveness is inversely associated with preclinical medical students&#x2019; anxiety [<xref ref-type="bibr" rid="ref8">8</xref>]. Although confidence typically improves with training, faculty and administrators can implement proactive strategies and training to support students&#x2019; interpersonal and professional development. Future research should explore longitudinal trends to refine educational interventions that enhance assertiveness and mental well-being.</p></sec></sec></body><back><ack><p>We thank Coretta Jenerette, PhD, RN, for permitting us to use the Simple Rathus Assertiveness Scale-Short Form (SRAS-SF).</p></ack><notes><sec><title>Data Availability</title><p>The data used to support our conclusions were not acquired from a public repository. The raw data and statistical analyses can be accessed through openICPSR [<xref ref-type="bibr" rid="ref11">11</xref>].</p></sec></notes><fn-group><fn fn-type="con"><p>Conceptualization: J Shaw, JH, AL, KM, AEW, J Shin, VL, BP, CL, PB, AJ</p><p>Data curation: J Shaw</p><p>Formal analysis: J Shaw</p><p>Investigation: J Shaw, JH, AL, KM, AEW, J Shin, VL, BP, CL, PB, AJ</p><p>Methodology: J Shaw, AJ</p><p>Project administration: J Shaw</p><p>Supervision: AJ</p><p>Visualization: J Shaw, AL</p><p>Writing &#x2013; original draft: J Shaw, JH, KM, AEW, J Shin, VL, BP, CL, PB</p><p>Writing &#x2013; review &#x0026; editing: J Shaw, JH, AL, AJ</p></fn><fn fn-type="conflict"><p>None declared.</p></fn></fn-group><glossary><title>Abbreviations</title><def-list><def-item><term id="abb1">GAD-7</term><def><p>General Anxiety Disorder 7-item</p></def></def-item><def-item><term id="abb2">SRAS-SF</term><def><p>Simple Rathus Assertiveness Scale-Short 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